PLAT 12-well liquid spreader
$850.00 PLAT 12-well liquid spreader
3D
Published 2019-04-11T07:57:36+00:00
Application:
- Reduce antibody costs by up to 10-fold
- Perform IF staining with 5-20 µl of antibody/blocking solution per well
- Perform FISH with 20 µl volumes
- Enables whole plate staining on glass-bottomed plates
Parameters:
Material: PLA
Operating temperature: < 150 °C
Autoclavable: No
Clean using 70% and 100% ethanol.
Distance between well centres: 26 mm
Design basis: Greiner Bio-one Cellstar 12-well plate
Protocol:
Minor modifications to staining protocols may be necessary. During testing, we established that the columns retain heat after FISH incubation for 10 min at 80 °C. The plate should be immediately cooled at 4 °C to prevent slow reannealing of DNA and to improve signal to noise ratio. More stringent post-FISH wash with lower SSC buffer concentration should be performed to reduce cytoplasmic background staining.
Modifications:
Users may attach glass coverslips or aluminium foil to the base of each column to create a flatter surface and promote ease of cleaning.
| Date published | 11/04/2019 |
| Price | $850.00 |
| Support Free | YES |