- Reduce antibody costs by up to 10-fold
- Perform IF staining with 5-20 µl of antibody/blocking solution per well
- Perform FISH with 20 µl volumes
- Enables whole plate staining on glass-bottomed plates
Operating temperature: < 150 °C
Clean using 70% and 100% ethanol.
Distance between well centres: 9 mm
Design basis: Corning Costar 96-well plate
Minor modifications to staining protocols may be necessary. During testing, we established that the columns retain heat after FISH incubation for 10 min at 80 °C. The plate should be immediately cooled at 4 °C to prevent slow reannealing of DNA and to improve signal to noise ratio. More stringent post-FISH wash with lower SSC buffer concentration should be performed to reduce cytoplasmic background staining.
Users are not advised to modify the columns to ensure that air is not trapped between the column and well.