PLAT 96-well liquid spreader
$850.00 PLAT 96-well liquid spreader
3D
Published 2019-04-11T07:57:24+00:00
Application:
- Reduce antibody costs by up to 10-fold
- Perform IF staining with 5-20 µl of antibody/blocking solution per well
- Perform FISH with 20 µl volumes
- Enables whole plate staining on glass-bottomed plates
Parameters:
Material: PLA
Operating temperature: < 150 °C
Autoclavable: No
Clean using 70% and 100% ethanol.
Distance between well centres: 9 mm
Design basis: Corning Costar 96-well plate
Protocol:
Minor modifications to staining protocols may be necessary. During testing, we established that the columns retain heat after FISH incubation for 10 min at 80 °C. The plate should be immediately cooled at 4 °C to prevent slow reannealing of DNA and to improve signal to noise ratio. More stringent post-FISH wash with lower SSC buffer concentration should be performed to reduce cytoplasmic background staining.
Modifications:
Users are not advised to modify the columns to ensure that air is not trapped between the column and well.
| Date published | 11/04/2019 |
| Precio | $850.00 |
| Sin apoyo | YES |